报告题目：Clearing of heat-stress induced protein aggregates requires a functional TPLATE complex

报  告  人：Daniel Van Damme教授（Ghent University）

时        间：2019年10月14日（星期一）10:00—12:00

地        点：学研大厦A0112

报告人简介：

    Daniel Van Damme教授，2001年就读于根特大学，并于2006年获得博士学位。2012年成为VIB Expert Scientist。2016年成为VIB group leader。其主要研究方向为植物细胞定向分裂和植物胞吞作用。Van Damme组发现了植物特有的胞吞相关组件TPLATE复合体，为深入研究植物胞吞过程做出了重要的贡献。目前，在Cell、PNAS、Nature Communication等期刊发表了多篇有影响力的学术论文。

报告摘要：

Clathrin-mediated endocytosis (CME) is the predominant endocytic pathway to regulate plasma membrane protein turnover in plants. The octameric TPLATE complex (TPC) serves as an essential adaptor complex during plant CME and functions in actin-dependent autophagy. Here, we destabilized the TPC subunit TPLATE by mutating an evolutionary conserved motif. We termed this destabilized form of TPLATE, WDX. We show that TPC harboring WDX is hypersensitive to short term heat stress, causing seedling lethality. WDX and autophagy mutants exhibited opposite phenotypes under heat stress and carbon starvation conditions. Heat stress-induced aggregated as well as ubiquitinated proteins accumulated heavily in the absence of a functional TPC and this did not rely on the autophagosomal degradation pathway activated during carbon starvation. Protein clearance during heat stress therefore relies more on a functional TPC rather than a functional ATG5/7-dependent autophagy pathway.

欢迎各位老师、同学参加！

报告联系人：李晓娟 教授