研究方向：

植物分子细胞与树木发育学；树木生命活动多模态跨尺度成像与分析；植物氮素摄取与根系形态建成；植物膜蛋白参与调控发育与逆境

学习、工作经历：

2021.07–至今，北京林业大学，生物科学与技术ky开云（中国），讲师，硕士生导师

2018.04–2021.07，北京林业大学，生态与自然保护ky开云（中国），博士后

2013.09–2017.12，北京林业大学，植物学，博士

2010.09 至 2013.07，河南科技大学，植物学，硕士

承担项目：

（1）科技部农业生物育种重大项目子课题，抗松材线虫病油松分子育种技术与新种质创制，70万元，在研，主持

（2）北京市自然科学基金面上项目，基于多尺度三维重构与单细胞时空ky开云手机版分析的银杏种胚成熟机制研究，20万元，在研，主持

（3）国家自然科学基金项目，基于多尺度三维重构与拓扑分析的种子休眠与发育调控机制研究，24万元，结题，主持

（4）科技部农业生物育种重大项目，抗松材线虫病高产油松新品种设计与培育，270万元，在研，参与

（5）国家自然科学基金重点项目，磷酸化调控重要膜蛋白ky开云手机版及其功能的机制研究，296万元，在研，参与

（6）中央高校基本科研业务费专项资金，针叶树重要性状的遗传调控解析与关键育种技术研发，240万元，在研，参与

（7）中央高校基本科研业务费专项资金，基于多尺度三维重构与单细胞转录分析的银杏种胚成熟机制研究，5万元，在研，主持

（8）中国博士后科学基金面上项目，NRT1.1磷酸化修饰调控气孔开闭的分子细胞学机制研究，8万元，结题，主持

（9）国家自然科学基金项目，NRT1.1磷酸化修饰调控植物侧根发育的分子细胞学机制研究，59万元，结题，参与

（10）国家自然科学基金项目，拟南芥CJP1蛋白参与茉莉素调控的诱导型抗性的分子机理研究，26万元，结题，参与

成果与奖励：

国家林业和草原局重点推广林草科技成果（2021年，成果名称：树木良种的“复幼-扦插”规模化繁育技术）

论文入选全球生物医学领域权威同行评议数据库Faculty Opinions，评分9.3（2021年，论文：Zhang et al., 2019, Science China-Life Sciences）

中关村NMT联盟“非损伤微测技术应用贡献奖”（2021年）

北京林业大学青年学术论文奖三等奖（2021年）

北京林业大学中央高校优秀青年团队（2023年）

北京林业大学2023年教育教学改革与研究优秀论文奖一等奖、三等奖、优秀奖（2023年）

北京林业大学第二十届青年教师教学基本功比赛三等奖（2024年）

发表文章情况：

1. Yuchen Kou#, Bodan Su#, Shunyao Yang, Wei Gong, Xi Zhang* (张曦) and Xiaoyi Shan*. Phosphorylation of Arabidopsis NRT1.1 regulates plant stomatal aperture and drought resistance in low nitrate condition. BMC Plant Biology, 2025, 25, 95.

2. Zijian Hu#, Jiazheng Liu#, Shiya Shen#, Weiqian Wu, Jingbin Yuan, Weiwei Shen, Lingyu Ma, Guangchao Wang, Shunyao Yang, Xiuping Xu, Yaning Cui, Zhenchen Li, Lijun Shen, Linlin Li, Jiahui Bian, Xi Zhang* (张曦), Hua Han*, Jinxing Lin*. Large-volume fully automated cell reconstruction generates a cell atlas of plant tissues, Plant Cell, 2024, 36(12), 4840–4861.

3. Lingyu Ma#, Zijian Hu#, Weiwei Shen, Yingying Zhang, Guangchao Wang, Bang Chang, Jinkai Lu, Yaning Cui, Huimin Xu, Yun Feng, Biao Jin, Xi Zhang* (张曦), Li Wang*, Jinxing Lin*. 3D reconstruction and multi-omics analysis reveal a unique pattern of embryogenesis in Ginkgo biloba. Plant physiology 2024, 196(1), 95–111.

4. Sammar Abbas, Ruotong Jing, Manzar Abbas, Zijian Hu, Rabia Kalsoom, Syed Sarfaraz Hussain, Liang Du, Jinxing Lin and Xi Zhang* (张曦). Transcriptional profiling of BpWRKY49 reveals its role as a master regulator in stress signaling pathways in Birch (Betula platyphylla). Forests 2024, 15, 605.

5. Yanhui Yuan#, Yi Li#, Siying Liu a, Pichang Gong b, Jinxing Lin and Xi Zhang* (张曦). An overview of aptamer: Design strategy, prominent applications, and potential challenge in plants. Journal of Plant Physiology 2024, 296, 154235.

6. Yi Li#, Yanhui Yuan#, Zijian Hu, Siying Liu, Xi Zhang* (张曦). Genetic transformation of forest trees and its research advances in stress tolerance. Forests 2024, 15, 441.

7. Xi Zhang# (张曦), Yi Man#; Xiaohong ZhuangX#. Jinbo Shen#, Yi Zhang#, Yaning Cui et al. Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques. Science China-Life Sciences 2021, 64, 1392–1422.

8. Xi Zhang# (张曦), Zijian Hu, Yayu Guo, Xiaoyi Shan, Jinxing Lin*. High-efficiency procedure to characterize, segment, and quantify complex multicellularity in raw micrographs in plants. Plant Methods 2020, 16, 100.

9. Pingli Liu#, Xi Zhang# (张曦Co-first author), Jianfeng Mao, Yanming Hong, Rengang Zhang Shuai Nie et al. The Tetracentron genome provides insight into the early evolution of eudicots and the formation of vessel elements. Genome Biology. 2020, 21, 291.

10. Xi Zhang# (张曦), Yaning Cui, Meng Yu, Bodan Su, Wei Gong, F Baluska, G Komis, J Samaj, Xiaoyi Shan*, Jinxing Lin*. Phosphorylation-mediated dynamics of nitrate transceptor NRT1.1 regulate auxin flux and nitrate signaling in lateral root growth. Plant Physiology. 2019, 181, 480–498.

11. Xi Zhang# (张曦), Yaning Cui, Meng Yu, Jinxing Lin*. Single-molecule techniques for imaging exo-endocytosis coupling in cells. Trends in Plant Science 2019, 24, 879–880.

12. Yaning Cui#, Xi Zhang# (张曦Co-first author), Meng Yu, Yingfang Zhu, Jingjing Xing, Jinxing Lin*. Techniques for detecting protein-protein interactions in living cells: principles, limitations, and recent progress. Science China-Life Sciences 2019, 62, 619–632.

13. Shaofeng Qu, Xi Zhang# (张曦Co-first author), Yutong Song, Jinxing Lin, Xiaoyi Shan*. THESEUS1 positively modulates plant defense responses against Botrytis cinerea through GUANINE EXCHANGE FACTOR4 signaling. Journal of Integrative Plant Biology 2017, 59, 797–804.

14. Yushuang Song#, Keji Yu#, Shuwen Zhang, Yi Li, Changwen Xu, Hongping Qian, Yaning Cui, Yayu Guo, Xi Zhang (张曦), Ruili Li, Richard A. Dixon, Jinxing Lin*. Poplar glutathione S-transferase PtrGSTF8 contributes to reactive oxygen species scavenging and salt tolerance. Plant Physiology and Biochemistry. 2024, 212 108766.

15. Weiwei Shen, Chen Zhang, Guangchao Wang, Yujian Li, Xi Zhang (张曦), Yaning Cui, Zijian Hu, Shiya Shen, Xiuping Xu, Yuan Cao, Xiaojuan Li, Jialong Wen*, Jinxing Lin*. Variation pattern in the macromolecular (cellulose, hemicelluloses, lignin) composition of cell walls in Pinus tabulaeformis tree trunks at different ages as revealed using multiple techniques. International Journal of Biological Macromolecules. 2024, 268 131619.

16. Yujian Li†, Weiwei Shen†, Xi Zhang (张曦),Yaning, Yuanyuan Zhao, Yayu Guo, Xiaojuan Li, Shuizhong Wang, Guoyong Song, Pu Wang, Jianfeng Ma, Jinxing Lin*. Single-cell characterization of major components of plant cell walls in situ by Raman spectroscopy. Science China-Life Sciences 2024, 67(8), 1772–1774.

17. Li, R.; Zhao, R.; Yang, M.; Xi Zhang (张曦); Lin, J. Membrane microdomains: structural and signaling platforms for establishing membrane polarity. Plant Physiology 2023, 1–18.

18. Li, R.; Xu, J.; Qi, Z.; Zhao, S.; Zhao, R.; Ge, Y.; Li, R.; Kong, X.; Wu, Z.; Xi Zhang (张曦) et al. High-resolution genome mapping and functional dissection of chlorogenic acid production in Lonicera maackii. Plant Physiology 2023, 192, 2902–2922.

19. Guo, Y.; Wang, S.; Yu, K.; Wang, H.L.; Xu, H.; Song, C.; Zhao, Y.; Wen, J.; Fu, C.; Li, Y.; Wang, S.Z.; Xi Zhang (张曦) et al. Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar. Nature Communication 2023, 14, 4285.

20. Cui, Y.; Xi Zhang (张曦); Li, X.; Lin, J. Multiscale microscopy to decipher plant cell structure and dynamics. New Phytologist. 2023, 237, 1980–1997.

21. Xu, C.; Abbas, S.; Qian, H.; Yu, M.; Xi Zhang (张曦); Li, X.; Cui, Y.; Lin, J. Environmental cues contribute to dynamic plasma membrane organization of nanodomains containing Flotillin-1 and hypersensitive induced Reaction-1 Proteins in Arabidopsis thaliana. Frontiers in Plant Science 2022, 13, 897594.

22. Pang, L.; Ma, Z.; Xi Zhang (张曦); Huang, Y.; Li, R.; Miao, Y.; Li, R. The small GTPase RABA2a recruits SNARE proteins to regulate the secretory pathway in parallel with the exocyst complex in Arabidopsis. Molecular Plant 2022, 15, 398–418.

23. Xing, J.; Duan, Z.; Zhang, L.; Xi Zhang (张曦); Lin, J. 3D imaging of lipid-guided vesicle trafficking along the cytoskeleton. Trends in Plant Science 2021, 26, 421–422.

24. Su, B.; Xi Zhang (张曦); Li, L.; Abbas, S.; Yu, M.; Cui, Y.; Baluska, F.; Hwang, I.; Shan, X.; Lin, J. Dynamic spatial reorganization of BSK1 complexes in the plasma membrane underpins signal-specific activation for growth and immunity. Molecular Plant 2021, 14, 588–603.

25. Yu, M.; Li, R.; Cui, Y.; Chen, W.; Li, B.; Xi Zhang (张曦); Bu, Y.; Cao, Y.; Xing, J.; Jewaria, P.K.; et al. The RALF1-FERONIA interaction modulates endocytosis to mediate control of root growth in Arabidopsis. Development 2020, 147, dev189902.

26. Yu, M.; Cui, Y.; Xi Zhang (张曦); Li, R.; Lin, J. Organization and dynamics of functional plant membrane microdomains. Cellular and Molecular Life Sciences 2020, 77, 275–287.

27. Shen, W.; Ma, L.; Xi Zhang (张曦); Li, X.; Zhao, Y.; Jing, Y.; Feng, Y.; Tan, X.; Sun, F.; Lin, J. Three-dimensional reconstruction of Picea wilsonii Mast. pollen grains using automated electron microscopy. Science China-Life Sciences 2020, 63, 171–179.

28. Liu, L.; Wang, Z.; Li, J.; Xi Zhang (张曦); Wang, R. A non-invasive analysis of seed vigor by infrared thermography. Plants 2020, 9, 768.

29. Li, L.; Su, B.; Qi, X.; Xi Zhang (张曦); Song, S.; Shan, X*. JA-induced endocytosis of AtRGS1 is involved in G-Protein mediated JA responses. International Journal of Molecular Sciences 2019, 20, 3779.

30. Guo, D.L.*; Hou, X.G.*; Xi Zhang (张曦). A simple and efficient method to isolate LTR sequences of plant retrotransposon. BioMed Research International 2014, 2014, 658473.

31. 李奕#, 张曦# (共同第一作者), 袁艳辉, 公丕昌*, 林金星* (2023), 适配体技术及其在植物科学研究中的应用. 植物学报 58(6): 1–11.

32. 杨舜垚#, 张贵芳#, 张曦# (共同第一作者), 林昌明, 黄婷, 邓乐平, 张智霖, 李福明, 钟岁英, 盘晓莺, 何其邹洪, 张莹莹, 鄂一岚, 沈微微, 王广超, 李洋, 赵媛媛, 李晓娟, 黄少伟, 沈应柏, 钮世辉*, 林金星* (2023) 湿加松个体老化模式与平茬复幼的机理. 中国科学: 生命科学 53(8): 1146–1165.

33. 马灵玉#, 祁晓红#, 胡子建, 沈微微, 王广超, 张柏林, 张曦*, 林金星* (2022) 光学透明技术在植物成像与细胞生物学中的应用. 植物学报 57(1): 98–110.

34. 张曦, 林金星, 单晓昳* (2016) 拟南芥无机氮素转运蛋白及其磷酸化调控研究进展. 植物学报 51(1): 120–129.

35. 张曦, 侯小改*, 郭大龙*, 宋程威. (2014) 利用iPBS技术克隆牡丹反转录转座子LTR序列. 植物学报 49(3): 322–330.

36. 徐昌文, 钱虹萍, 罗鹏云, 玉猛, 邢晶晶, 张亮, 张曦, 崔亚宁*, 林金星* (2023) 植物膜蛋白的囊泡转运及调控机制的研究进展. 科学通报 68(7):762–778.

37. 李雨健, 沈微微, 殷金环, 张曦, 崔亚宁, 赵媛媛, 林金星* (2022) 多炔彩虹探针在生物学研究中的应用与展望. 中国细胞生物学学报 44(12): 2375–2385.

38. 古盼, 齐学影, 李莉, 张曦, 单晓昳* (2022) AtRGS1胞吞ky开云手机版调控G蛋白参与拟南芥生长发育和抗性反应. 生物技术通报 38(6): 34–42.

39. 马灵玉, 王广超, 张曦, 胡子健, 沈微微, 林金星* (2021) 拟南芥子叶发育过程中叶绿体特征的量化及分布分析. 电子显微学报 40(3): 270–278.

40. 宫伟, 余健源, 张曦, 单晓昳* (2020) 硝酸根调控植物开花和产量分子机制的研究进展. 生物技术通报 36(8): 167–172.

41. 崔亚宁, 满奕, 宋程威, 张曦, 钱虹萍, 林金星* (2020) 植物凯氏带化学成分、生理功能及相关调控机制的研究进展. 中国科学生命科学 50(2): 102–110.

42. 宋程威, 郭大龙, 张曦, 郭丽丽, 侯小改* (2014) 牡丹LINE 类反转录转座子RT 序列的克隆及分析. 园艺学报 41(1): 157–164.

43. 侯小改#*, 郭大龙#, 黄燕梅, 张曦 (2013) 牡丹 Ty3-gypsy 类反转录转座子反转录酶序列的克隆及分析. 园艺学报 40(1): 98–106.

44. 侯小改*, 张曦, 郭大龙* (2012) 植物 LTR 类反转录转座子序列分析识别方法. 遗传 34(11): 1491–1500.